E.coli "strain 3" G235E CheA on

chromosomal chemoreceptor mutant G235E "CheA on"<strong>Tilt Series Date:</strong> 2012-02-10</p> <strong>Data Taken By:</strong> Ariane Briegel</p> <strong>Species / Specimen:</strong> Escherichia coli</p> <strong>Strain:</strong> RP 437</p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x. </p> <strong>Acquisition Software:</strong> UCSFTomo</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> Raptor</p> <strong>Collaborators and Roles:</strong> Sandy Parkinson</p> <strong>Purification / Growth Conditions / Treatment:</strong> culture grown in TB medium (+GEntamycin) to OD (600) 0.31 at 30 degrees. Additional one hour incubation with penicillin before freezing.</p> <strong>Sample Preparation:</strong> freezing with Vitrobot @ 100 % humidity, -4 offset, 3.5 s blot time, no drain, 3 µl sample, pure ethane.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-10-8</p>strain30008.mrc, Tilt Series (Pixel Size 0.644 nm), 1.1 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-10-8/rawdata/strain30008.mrc" > <i class="download icon"></i> Download </a></p> strain30008_part.rec, Reconstruction (Pixel Size 1.288 nm), 516.1 MB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-10-8/3dimage_16356/strain30008_part.rec" > <i class="download icon"></i> Download </a></p&gt